uterine smooth muscle utsmc cell line  (PromoCell)

Journal: Molecular human reproduction

Article Title: Overexpression of the truncated form of High Mobility Group A proteins (HMGA2) in human myometrial cells induces leiomyoma-like tissue formation.

doi: 10.1093/molehr/gau114

Figure Lengend Snippet: Figure2 ExpressionofHighMobilityGroupAproteins(HMGA2)inhumanmyometrialcells.(A)ComparativeexpressionpatternsofHMGA2-full/long versus HMGA2-truncated/short in human myometrial cells (hMyo) and leiomyoma (Leio) derived cells (LeioSP, LeioNSP, LeioTF) and (B) leiomyoma SP cell lines (LeioSP1 and LeioSP2). The human embryonic stem cell line VAL-5 (hESC VAL5) was used as a positive control for HMGA2 expression and water (no RNA samples) wasused as anegative control(2). NB: SP, side population; NSP, non-side population; TF, unsorted leiomyomacells; SP1/2 ¼ cell lines previouslyderivedfromLeioSPcells.DetectionbyRT–PCRofHMGA2-truncated/shortexpression(SF)versusHMGA2-full/long(LF)aftertransfectionin (C) myometrial primary cells (hMyo), (D) myometrial cell line (hUtSMC) and (E) myometrial SP (MyoSP) cells. In these panels, the Y-axis shows fold ex- pression relative to hESC VAL5 cells (VAL). NB: C- ¼ untransfected; Ev ¼ transfected with empty vector.

Article Snippet: Cell culture conditions and transfection experiments Primary human myometrial cells (hMyo), myometrial Side Population cells (MyoSP) and a human uterine smooth muscle cell line (hUtSMC) (Promocell, C12575, Germany) were grown in supplemented medium in a hypoxic environment (2% O2, 378C, 5% CO2, 90% humidity) (Cervelló et al., 2011; Mas et al., 2012).

Techniques: Derivative Assay, Positive Control, Expressing, Control, Transfection, Plasmid Preparation

Journal: Molecular human reproduction

Article Title: Overexpression of the truncated form of High Mobility Group A proteins (HMGA2) in human myometrial cells induces leiomyoma-like tissue formation.

doi: 10.1093/molehr/gau114

Figure Lengend Snippet: Figure 3 Growth properties of cells transfected with different High Mobility Group A proteins (HMGA2) vectors. (A) Images comparing colonies trans- formed withempty vector(Ev; control) orthe long (LF) or short form (SF) ofHMGA2 growing insemisolid soft agarculturemedium.(All images weretaken at 40-fold magnification.) Transforming properties of cells transfected with LF, SF or Ev of HMGA2 by CE (B, D and F) and soft agar assays (C, E and G). Myometrial primary cells (hMyo) (B and C), myometrial cell line (hUtSMC), (D and E) and myometrial Side Population (MyoSP) cells (F and G).

Article Snippet: Cell culture conditions and transfection experiments Primary human myometrial cells (hMyo), myometrial Side Population cells (MyoSP) and a human uterine smooth muscle cell line (hUtSMC) (Promocell, C12575, Germany) were grown in supplemented medium in a hypoxic environment (2% O2, 378C, 5% CO2, 90% humidity) (Cervelló et al., 2011; Mas et al., 2012).

Techniques: Transfection, Plasmid Preparation, Control